技巧从GFF⽂件提取CDS并翻译成蛋⽩Usage:
gffread <input_gff> [-g <genomic_seqs_fasta> | <dir>][-s <seq_info.fsize>]
[-o <outfile.gff>] [-t <tname>] [-r [[<strand>]<chr>:]<start>..<end> [-R]]
[-CTVNJMKQAFGUBHZWTOLE] [-w <exons.fa>] [-x <cds.fa>] [-y <tr_cds.fa>]
[-i <maxintron>]
Filters and/or converts GFF3/GTF2 records.
<input_gff> is a GFF file, use '-' if the GFF records will be given at stdin
Options:
-g  full path to a multi-fasta file with the genomic sequences
for all input mappings, OR a directory with single-fasta files
(one per genomic sequence, with file names matching sequence names)
-
s  <seq_info.fsize> is a tab-delimited file providing this info
for each of the mapped sequences:
<seq-name> <seq-length> <seq-description>
(useful for -A option with mRNA/EST/protein mappings)
-i  discard transcripts having an intron larger than <maxintron>
-r  only show transcripts overlapping coordinate range <start>..<end>
(on chromosome/contig <chr>, strand <strand> if provided)
-R  for -r option, discard all transcripts that are not fully
contained within the given range
-U  discard single-exon transcripts
-C  coding only: discard mRNAs that have no CDS featureconversion翻译方法的定义
-
F  full GFF attribute preservation (all attributes are shown)
-G  only parse additional exon attributes from the first exon
and move them to the mRNA level (useful for GTF input)
-A  use the description field from <seq_info.fsize> and add it
as the value for a 'descr' attribute to the GFF record
-O  process also non-transcript GFF records (by default non-transcript
records are ignored)
-V  discard any mRNAs with CDS having in-frame stop codons
-H  for -V option, check and adjust the starting CDS phase
if the original phase leads to a translation with an
in-frame stop codon
-
B  for -V option, single-exon transcripts are also checked on the
opposite strand
-N  discard multi-exon mRNAs that have any intron with a non-canonical
splice site consensus (i.e. not GT-AG, GC-AG or AT-AC)
-J  discard any mRNAs that either lack initial START codon
or the terminal STOP codon, or have an in-frame stop codon
(only print mRNAs with a fulll, valid CDS)
--no-pseudo: filter out records matching the 'pseudo' keyword
-M/--merge : cluster the input transcripts into loci, collapsing matching
transcripts (those with the same exact introns and fully contained)
-d <dupinfo> : for -M option, write collapsing info to file <dupinfo>
-
-cluster-only: same as --merge but without collapsing matching transcripts
-K  for -M option: also collapse shorter, fully contained transcripts
with fewer introns than the container
-Q  for -M option, remove the containment restriction:
(multi-exon transcripts will be collapsed if just their introns match,
while single-exon transcripts can partially overlap (80%))
--force-exons: make sure that the lowest level GFF features are printed as
"exon" features
-E  expose (warn about) duplicate transcript IDs and other potential
problems with the given GFF/GTF records
-D  decode url encoded characters within attributes
-
Z  merge close exons into a single exon (for intron size<4)
-w  write a fasta file with spliced exons for each GFF transcript
-x  write a fasta file with spliced CDS for each GFF transcript
-W  for -w and -x options, also write for each fasta record the exon
coordinates projected onto the spliced sequence
-y  write a protein fasta file with the translation of CDS for each record
-L  Ensembl GTF to GFF3 conversion (implies -F; should be used with -m)
-m  <chr_replace> is a reference (genomic) sequence replacement table with
this format:
<original_ref_ID> <new_ref_ID>

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