Contents of ImageQuant TL Quick Start
•Introduction to ImageQuant TL and Installation Instructions
•The Control Centre – Getting Started
•The ImageQuant TL Interface
•1D Gel Analysis
•Array Analysis
•Colony Counter Analysis
•General Image Analysis – Toolbox Module
Introduction
This booklet provides a quick introduction to ImageQuant TL and guides you through the analysis process in each module. The figures that accompany each section help you work through your analysis and relate to the provided tutorial images.
This Quick Start does not describe all the features in ImageQuant TL. For more information view the online Help available within the software. If you have any questions or feedback on ImageQuant TL, please contact Amersham Biosciences Technical Support at iqtl@imsupport or your local Amersham Biosciences representative. ImageQuant TL Quick Start Page 2
Installation
Before starting the installation, you should close all open programs.
•Insert the CD and the installer should start automatically. If this option is disabled on your system, select Run from the Start menu. In the Run window, type d:\, where “d” is the letter that corresponds to your CD drive.
•Follow the instructions that appear on the screen.
The Control Centre – Getting Started
To start ImageQuant TL, double-click the ImageQuant TL icon on the desktop. Alternatively, select ImageQuant TL v2003 from the Start/Programs menu. The Control Centre window appears.
Opening an Image
ImageQuant TL can analyse 8- to 16-bit greyscale Tiff images, .gel files, and .ds files Array from most types of image capture devices currently available.
To open an image in an analysis module click the button in the Control Centre, or if
already in the analysis module, select Open from the File menu. The Open Image
window appears.
The demonstration images are located in the Images folder of the ImageQuant TL
program group. Select the image and the analysis module you want to use and click
on Open.
The software automatically saves your analysis throughout the process.
ImageQuant TL can perform a full analysis of multichannel images (.ds format). When a multichannel image is
loaded, the interface changes to allow access to the multichannel analysis features.**
The Overlay and Single buttons on the main tool bar allow you to select the image view. If you select Overlay, you choose the channels to be viewed using the channel selector buttons. This determines w
hich channel data appears in the main image window, the lane profile window and the measurements table.
To assist with the analysis, you can access a second image window using the buttons under the Navigator. The second image window allows you to visually compare the channels you select using the buttons in the window. If you want to edit the image, you use the main image window. The software updates the second image window automatically.
Refer to the Help pane throughout the analysis process, or refer to the footnotes throughout this document that describe how an analysis would be carried out on a multichannel image.
** Colony counter module cannot load multichannel images
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ImageQuant TL Quick Start  Page 4 The ImageQuant TL Interface
The General Layout
The appearance of the program remains the same throughout each analysis module.
A toolbar appears at the top of the window that contains the following buttons.
Additional windows, such as the Image View, Analysis, Measurements Table, MW Curve, Report and Second Image Window (to be used when analysing multichannel images) can be selected from the buttons at the bottom of the Navigator.
The Navigator
The pane at the left of the screen is the Navigator. The Navigator contains
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the tools required for the current mode of the analysis.
ImageQuant TL uses a wizard interface; Next  and Previous  buttons allow
easy navigation through the analysis modes. The Restart  button allows you to
begin the analysis again
A Help pane is also present that provides information and tips relevant to the
current analysis.
Click the Parameters  tab to view any additional parameters.
Image viewing tools
You access the image viewing tools from the buttons on the Image window.
Zoom: Drag a rectangle on the image window, and then left-click to increase or right-click to decrease the image magnification. Alternatively, you can drag the scroll bar edges.
Zoom 1:1: View the image at a 1:1 ratio.
Fit to window: View the whole image.
Magnify: Allows magnification of a portion of the image.
Panning: The image can be moved around the image window when part of the image is not visible, if for example the zoom tool has been used.
Contrast
The contrast window allows you to improve the way the image is displayed. The values are based on the image calibration during scanning. The contrast can be altered in either the Single or Overlay view. When analysing in Overlay  you select the channels for contrast adjustment from the drop-down menu. The original data remains unaltered.
Colour
The colour window allows you to change the colour of the displayed image. You cannot make alterations to the colour display in the Overlay  view.
Edit Image
The image manipulation tools are available from the main tool bar and allow you to edit the image. All t
he edits are logged for a specific image and can be viewed in the Image Properties  window. Editing can be performed in either the Single or Overlay
view. All the edits appear in all the channels of a multichannel image.
Options
You can access a variety of options using the button on the main tool bar. These options are specific to the selected analysis module and allow customisation of the Image Window and Measurements Table.
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