Reporting Checklist For Life Sciences Articles
This checklist is used to ensure good reporting standards and to improve the reproducibility of published results. For more information, please read Reporting Life Sciences Research .
▸  Figure legends
•  Check here to confirm that the following information is available in all relevant figure legends (or Methods section if too long):
• t he exact sample size (n ) for each experimental group/condition, given as a number, not a range;
• a description of the sample collection  allowing the reader to understand whether the samples represent technical or biological replicates
(including how many animals, litters, culture, etc.);
• a statement of how many times the experiment shown was replicated in the laboratory ;
•  d efinitions of statistical methods and measures : (For small sample sizes (n<5) descriptive statistics are not appropriate, instead plot indi-
vidual data points)
o  v ery common tests, such as t -test, simple χ2 tests, Wilcoxon and Mann-Whitney tests, can be unambiguously identified by name only,
but more complex techniques should be described in the methods section;
o are tests one-sided or two-sided?
o are there adjustments for multiple comparisons?
comparisonso statistical test results , e.g., P  values ;
o definition of ‘center values ’ as median  or mean ;
o definition of error bars  as s.d. or c.i.
This checklist will not be published. Please ensure that the answers to the following questions are reported in the manuscript itself. We encourage you to include a specific subsection in the Methods section for statistics, reagents and animal models. Below, provide the page number or section and p
aragraph number (e.g. “Page 5” or “Methods, ‘reagents’ subsection, paragraph 2”).Corresponding Author Name:  ________________________________________Manuscript Number:  ______________________________▸  Statistics and  general methods Reported in section/paragraph or page #:1.  H ow was the sample size chosen to ensure adequate power to detect a pre-specified effect size? (Give section/paragraph or page #) For animal studies, include a statement about sample size estimate
even if no statistical methods were used.
2.  D escribe inclusion/exclusion criteria if samples or animals were
excluded from the analysis. Were the criteria pre-established?
(Give section/paragraph or page #)
3.  I f a method of randomization was used to determine how samples/
animals were allocated to experimental groups and processed,
describe it.  (Give section/paragraph or page #)
F or animal studies, include a statement about randomization even if no
randomization was used.
4.  I f the investigator was blinded to the group allocation during the
experiment and/or when assessing the outcome, state the extent of
blinding. (Give section/paragraph or page #)
For animal studies, include a statement about blinding even if no blinding
was done.
5.  F or every figure, are statistical tests justified as appropriate?
Do the data meet the assumptions of the tests (e.g., normal distribution)?
Is there an estimate of variation within each group of data?
Is the variance similar between the groups that are being statistically
compared?  (Give section/paragraph or page #)
6. T o show that antibodies were profiled for use in the system under
study (assay and species), provide a citation, catalog number and/or
clone number, supplementary information or reference to an antibody
validation profile (e.g., Antibodypedia, 1DegreeBio).
7. Cell line identity:
a. Are any cell lines used in this paper listed in the database of
commonly misidentified cell lines maintained by ICLAC (also
available in NCBI Biosample)?
b. If yes, include in the Methods section a scientific justification of
their use – indicate here on which page (or section and paragraph)
the justification can be found.
c. For each cell line, include in the Methods section a statement
that specifies:
- the source of the cell lines
- have the cell lines been authenticated? If so, by which method?
- have the cell lines been tested for mycoplasma contamination?
In this checklist, indicate on which page (or section and paragraph)
the information can be found.
▸Animal Models Reported in section/paragraph or page #:
8. Report species, strain, sex and age of animals
9. F or experiments involving live vertebrates, include a statement of
compliance with ethical regulations and identify the committee(s)
approving the experiments.
10. W e recommend consulting the ARRIVE guidelines (PLoS Biol. 8(6), e1000412,2010) to ensure that other relevant aspects of animal studies are
adequately reported.
▸Human subjects Reported in section/paragraph or page #:
11. Identify the committee(s) approving the study protocol.
12. I nclude a statement confirming that informed consent was obtained
from all subjects.
13. F or publication of patient photos, include a statement confirming
that consent to publish was obtained.
14. R eport the clinical trial registration number (v or
equivalent).
15. F or phase II and III randomized controlled trials, please refer to the
CONSORT statement and submit the CONSORT checklist with
your submission.
16. F or tumor marker prognostic studies, we recommend that you follow
the REMARK reporting guidelines.
17. P rovide accession codes for deposited data.
Data deposition in a public repository is mandatory for:
a. Protein, DNA and RNA sequences
b. Macromolecular structures
c. Crystallographic data for small molecules
d. Microarray data
Deposition is strongly recommended for many other datasets for which structured public repositories exist; more  details on our data policy are available here. We encourage the provision of other source data in supplementary information or in unstructured repositories such as Figshare and Dryad.  We encourage publication of Data Descriptors (see Scientific Data) to maximize data reuse
18.  I f computer code was used to generate results that are central to
the paper’s conclusions, include a statement in the Methods section
under “Code availability” to indicate whether and how the code
can be accessed. Include version information as necessary and any
restrictions on availability.

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