中国医药生物技术2018年10月第13卷第5期Chin Med Biotechnol, October 2018, V ol. 13, No. 5 431 Regulatory role of pinoresinol diglucoside in the ER/TGF-β1/Smads signaling pathways in
photo-aged HDF cells
GAO Hai-na, CAI Zhou-quan, LIN Ying, WEN Xia, SUN Hui-feng
【Abstract】
Objective To study the effect of pinoresinol diglucoside (PDG) on the ER/TGF-β1/Smads signaling pathways in photo-aged human dermal fibroblasts (HDF) cells.
Methods    A long wave ultraviolet (UV A) model was established to damage the HDF cells, and different concentrations of PDG and ER/TGF-β1/Smads signaling pathway inhibitors were added. The cell proliferation rate was detected by MTT, and the expression of signal transforming growth factor (TGF- β1), transduced molecule (Smad3) and type I collagen (COL1A1) expression were detected by RT-PCR and Western blot method.
Results Compared with the control group, the proliferation rate and the expression of TGF-β1, Smad3, COL1A1 mRNA and protein in the UV A irradiated model group were significantly decreased (P < 0.01).
Compared with the model group, the proliferation rate and TGF-β1, Smad3, and COL1A1 expression were significantly increased after PDG treatment (P < 0.01). Compared with the high concentration of PDG group, the expression of TGF-β1, Smad3, and COL1A1 expression were significantly decreased, respectively, upon the treatment with respective inhibitor (P < 0.01).
Conclusion PDG increased the expression of TGF- β1, Smad3 and COL1A1 in HDF cells after UV A irradiation, and this effect can be inhibited by TGF-β1, Smad3 and ER inhibitor.
【Key words】Pinoresinol diglucoside; Human dermal fibroblasts cells; Phyto-oestrogens; ER/TGF-β1/Smad3 signaling pathway
Author Affiliations: Sichuan Science City Hospital, Mianyang 621000, China (GAO Hai-na, CAI Zhou-quan, LIN Ying, WEN Xia); Heilongjiang University of Chinese Medicine, Harbin 150040, China (SUN Hui-feng)proliferation
Corresponding Author: SUN Hui-feng, Email: huifengsun@hotmail
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