葡萄糖脱氢酶法的英文
    Glucose Dehydrogenase Method.
    The glucose dehydrogenase method is a spectrophotometric assay for determining the concentration of glucose in a sample. It is based on the enzymatic reaction catalyzed by glucose dehydrogenase (GDH), which oxidizes glucose to gluconic acid and reduces NAD+ to NADH. The amount of NADH produced is stoichiometrically equivalent to the amount of glucose in the sample, and can be measured by its absorbance at 340 nm.
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    The reaction is carried out in a cuvette containing a buffer, GDH, NAD+, and the sample. The change in absorbance at 340 nm is monitored over time, and the rate of change is used to calculate the concentration of glucose in the sample.
    The glucose dehydrogenase method is a simple, rapid, and accurate method for determining the concentration of glucose in a variety of samples, including blood, urine, and food products. It is commonly used in clinical chemistry laboratories and in food analysis.
    Principle of the Method.
    The glucose dehydrogenase method is based on the following enzymatic reaction:
    Glucose + NAD+ + H2O → Gluconic acid + NADH + H+。
    The reaction is catalyzed by the enzyme glucose dehydrogenase (GDH). GDH is a flavoenzyme that contains flavin adenine dinucleotide (FAD) as its prosthetic group. FAD is a coenzyme that accepts and donates electrons, and it is essential for the catalytic activity of GDH.
    In the glucose dehydrogenase method, the reaction is carried out in a cuvette containing a buffer, GDH, NAD+, and the sample. The NAD+ serves as an electron acceptor for the reaction, and the NADH produced is stoichiometrically equivalent to the amount of glucose in the sample.
    The amount of NADH produced can be measured by its absorbance at 340 nm. NADH has a strong absorbance at 340 nm, while NAD+ does not. Therefore, the change in absor
bance at 340 nm is directly proportional to the concentration of NADH in the sample.
    Procedure.
    The glucose dehydrogenase method is typically performed in a spectrophotometer. The following steps are involved in the procedure:
    1. Prepare a reaction mixture containing the following components:
        Buffer.
        GDH.
        NAD+。
        Sample.
    2. Incubate the reaction mixture at 37°C for a specified period of time (usually 10-15 minutes).
    3. Measure the absorbance of the reaction mixture at 340 nm.
    4. Calculate the concentration of glucose in the sample using the following equation:
    Glucose concentration = (ΔA340 / εb)  (V / v)。
    Where:
    ΔA340 is the change in absorbance at 340 nm.
    εb is the extinction coefficient of NADH at 340 nm (6.22 mM-1 cm-1)。
    V is the total volume of the reaction mixture (usually 1 mL)。
    v is the volume of the sample added to the reaction mixture (usually 10 μL)。

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