Cold Spring Harbor Protocols Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN-γSecreting T Cells INTRODUCTION
The enzyme-linked immunospot (ELISPOT) assay measures the secretion intensity of effector mole-cules released by immune cells in response to ex vivo antigenic stimulation, as well as the frequency
of these responding cells. This assay is highly sensitive, quantitative, easy to use, and amenable to high-throughput screening. For these reasons, the ELISPOT assay is considered by many as a gold standard for monitoring cellular immune responses. Until recently, ELISPOT assays using chro-mophores to detect the T cell secretion of cytokines were limited to the characterization of a single
effector molecule. Notably, studies evaluating the immune response to chronic viral infections often
measured IFN-γ secretion by ELISPOT because of the known antiviral effects of this cytokine as well as
its correlation to the cytotoxic capacity of T cells. However, maintenance of both IFN-γ and IL-2 secre-tion by pathogen-specific T cells has been linked to a more favorable clinical outcome in humanspring是什么意思cold
immunodeficiency virus (HIV) and Leishmania infections. Therefore, an ELISPOT assay able to simulta-neously characterize T cell responses in terms of IL-2 and IFN-γ secretion is potentially relevant for the
monitoring of immune responses to certain infectious agents. In this protocol, we describe an ELISPOT
assay for the simultaneous detection of IL-2 and IFN-γ upon stimulation with viral peptides.

版权声明：本站内容均来自互联网，仅供演示用，请勿用于商业和其他非法用途。如果侵犯了您的权益请与我们联系QQ:729038198，我们将在24小时内删除。