产品名称：人肝细胞(HH)
英文名称：Human Hepatocytes
产品分类：人类细胞株[Human Cell strain]
产品编号：C5200h
检验方法：
包 装：1株
产品说明：人肝细胞(HH) 概述
Cell Specification
The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, biotransformation of xenobiotics, and synthesis of plasma proteins under physiological and pathophysiological conditions [1]. Primary culture of human hepatocytes appears to be a suitable experimental model for the study of liver specific function, have been and still are an important tool. Propagation of human hepatocytes for cell transplantation, gene therapy, and culture of hepatocytes in bioartificial liver support systems is now under investigation [2]. In appropriate culture condition, cultured human hepatocytes proliferate and maintain differentiated hepatocyte function such as the synthesis of serum proteins [3].
HH from ScienCell Research Laboratories are isolated from human liver tissue. HH are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HH are characterized by immunofluorescent method with antibodies to albumin, cytokeratin-18 and vimentin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Hepatocyte Medium (HM, Cat. No. 5201) for the culturing of human hepatocytes in vitro.
Product Use
HH are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Runge, D., Michalopoulos, G. K., Strom, S. C. and Runge, D. M. (2000) Recent advances in human hepatocyte culture systems. Biochem. Biophysi. Res. Comm. 274:1-3.
[2] Chen, H. L., Wu, H. L., Fon, C. C., Chen, P. J., Lai, M. Y. and Chen, D. S. (1998) Long-term culture of hepatocytes from human adults. J. Biomed. Sci. 5:435-440.
[3] Okamoto, M., Ishida, Y., Keogh, A. and Strain, A. (1998) Evaluation of the function of primary human hepatocytes co-cultured with the human hepatic stellate cell (HSC) line LI90. Int. J. Artif. Organs 21:353-359.
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